Effect of Added Inoculum and Di-potasium hydrogen phosphate Concentration on the Fermentation of Dextran Production

Triantarti dan Hendro Santoso M

Indonesian Sugar Research Institute
Jl.Pahlawan 25 Pasuruan 67126
E-mail: isri@telkom.net


ABSTRACT

Dextran production is conducted by fermentation by using Leuconostoc mesenteriodes which produces dextransucrase enzyme. Sucrose is converted to dextran by dextransucrase. Sucrose is a main carbon source in dextran fermentation. Hence, sugar cane juice mainly contains sucrose is potential material for dextran fermentation. The effect of inoculum concentration added at the beginning of fermentation of di-potasium hydrogen phosphate concentration in the medium were studied. L. Mesenteriodes B-512F was used. The results showed that there were no effect on optimum growth and dextran production when the inoculum concentration added at 1% and 5% (v/v). The only difference was inoculum at 1% (v/v) delaying the growth and dextran formation in comparison to the addition of 5% (v/v) inoculum. The optimum growth and dextran production were affected by di-potasium hydrogen phosphate concentration in the medium (0,5 ; 1,0 and 1,5% w/v). The growth was highest at di-potasium hydrogen phosphate concentration 1,5% w/v. On the otherhand, dextran production was lower compared to the other treatments.

Keywords: dextran,fermentation,inoculum, di-potasium hydrogen phosphate.