Chromatographic Separation of Sugarcane Thick Juice
for High Fructose Syrup

Yahya Kurniawani dan Hendro Santoso M

Indonesian Sugar Research Institute
Jl.Pahlawan 25 Pasuruan 67126
E-mail: isri@telkom.net

ABSTRACT

This paper reports a study of chromatographic separation process of glucose-fructose mixtures. The experiments was conducted in laboratory scale using a column filled with 1800 ml of resin as stationary phase and aquades as mibile phase or eluent. The chromatographic separation was to study the fractionation of sugarcane thick juice after a preparation step which included decolorization, demineralization and inversion. Experiment conditions included the use of two resins (different in size) in the form of Calcium in 1000 ml column, feed volume 300 ml, temperature 550 C and flow rate 10 ml per minute and fractionation time of 10 minutes. The first treatment used below 50 mesh resin and the second between 50-100 mesh. The composition of glucose and fructose was determined using polarimetric method. Other analyses were done for brix using hand refractometer and Ph using Ph meter. Results showed that the glucose-fructose component of the juice was not so high in the first treatment but the separation was quite well in the second treatment. The results of the second treatment showed that chromatographic separation for the treated thick cane juice could produce good separation in both 65,30% brix glucose and 34,66% brix fructose mixture and in a high fructose content mixture composed of 47,26% brix glucose and 57,66% brix fructose. Therefore, it is posible to produce two kinds of products without isomerization step as follows: the first product was HFS 55 which contain 55% fructose and the second product was HFS 42 which contain 42% fructose. The analysis of these products showed that the quality could meet SNI standard except the ash content.

Keywords : chromatograpy, thick cane juice, hihg fructose syrup.


(MAJALAH IPTEK Vol.16, No. 2, Mei 2005)