Monday, October 27, 2008

The effect of Gibberellic acid and rice bran on the production of ethanol from cane molasses and sugarcane juice


Ma. Florencia T. Logrono, Ma. Lucia C. Sanchez, Ma. Adelfa A. Apoloinario, Marilou Agravante, Ma. Lourdes T. Escarrilla

Gibberellic acid and rice bran were observed to have stimulative effects on ethanol production using sugarcane juice and molasses as substrates. Gibberellic acid is known for its stimulative effect on the fermentative capacity of yeasts. Rice bran increases rate of ethanol fermentation and supplies nutrients which support yeast growth sufficiently during the process.
Saccharomyces cerevisiae and the feed yeast, Candida tropicales were utilized in this study. Fermentation of molasses and cane juice inoculated with S. cerevisiae supplemented with rice bran at 0.4-0.8% concentrations and 1-4 ppm gibberellic acid produced significantly higher alcohol than control. The same substrates inoculated with C. tropicales supplemented with the same concentrations of supplements yielded higher alcohol than control. Results on alcohol produced were comparable to each other but significantly different from control. All treatments were significant at 1% level over the control.
Rice bran supplemented molasses at 0.8% concentration inoculated with S. cerevisiae produced alcohol of 10.5% with corresponding fermentation efficiency of 94.95%. on the other hand, supplementation of 2 ppm gibberellic acid produced alcohol of 9.7% with 88.30% fermentation efficiency. The same concentrations of two supplements in sugarcane juice gave the highest yield of alcohol.
Addition of gibberellic acid and rice bran at 2 ppm and 0.8% concentrations, respectively enhanced alcohol production using molasses and cane juice as substrates. Increase in alcohol produced over control in rice bran and gibberellic acid supplemented molasses and cane juice ranged from 10-23%; 15-20%; 7-13%, respectively.

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Wednesday, October 22, 2008

The Use of Na and K-form Strong Acid Cation Resin for Chromatographic Separation of Sucrose from Cane Molasses

Hendro Santoso* and Theresia Hari Sutji W*
*Indonesia Sugar Research Institute
Jl.Pahlawan 25 Pasuruan Indonesia


The cane molasses is the residual syrup from sucrose crystallization process. It has sucrose content about 30-40 per cents and reducing sugar about 20-30 percents. Those sugar could be separated from molasses by using Ion Exclusion Chromatographic method. This paper describes the use of Na and K-form strong acid cation resin for separation of sucrose from cane molasses. The experiments was employed on two pilot scale chromatographic column. The A column with diameter of 40 cm and diameter of B column is 25 cm. Both of columns were contained 90 litres of Na-form resin (A column) and 150 litres of K-form resin (B column) with operating condition by flow rate 0,6 and 0,5 bed volum per hour respectively. The feed volume and temperature for both columns were 0,1 bed volume and 70oC. Pol,brix,sucrose, reducing sugar and Total Sugar As Invert (TSAI) were analyzed and evaluated. The results showed that the both of colomns system were improve the quality of sucrose fraction to be separated by increasing the purity of sucrose and decreasing of reducing sugar purity. On the A column system, sucrose purity compare to TSAI could increased up to 65,28% and with the B column system up to 67,77%. Furthere, reducing sugar content decreased up to 30,64% and 48,64% by using A and B column respectively. Sucrose Reducing sugar Ration could achieved 1,72 point by using B column system. It better than A column.

Key words : K and Na-cation resin, chromatography, sucrose fraction, cane molasses.

Sunday, October 19, 2008


Gading F Hutasoit

(Indonesian Sugar Research Institute, ISRI)


Wood demand has been increased in the last decade, while supply. One of the ways to meet demands is to look for a material as a wood substitute. The wood substitute should be cheap due to moneter and economic crisis. Particle board out of bagasse, is agood material for substitute wood. The acceptability of particle board as a wood substitute depend upon the quality, besides prices. There are many factors which affect the quality of particle board. The objective oh this research is to study the effect of bagasse fineness and proportion of glue on the quality of particle board. Bagasse was grinded and sieved. There were two types of bagasse which use in particle board making, they are coarse and fine fraction. Coarse fraction was retained in sieve of 14 mesh. Fine fractions was passed 14 mesh and retained in 28 mesh, while fraction passed 28 mesh was not used. Dry fraction was mixed with glue. The proportion of glue was varied they were 23 ; 28 ; 32 and 37% of dry weight fraction. Mixture of bagasse and glue was pressed. The physical and mechanical characters were measured. The result showed that characters of mixture of coarse fraction and 37% glue fulfilled the requirement of SII (Industrial Standard of Indonesia) 0797-83. The characters were density 0,55 g/cm3 ; thickness swelling (2 hours) 8%; thickness swelling (24 hours) 16% ; moisture 5,5% ; bending strength 121 kg/cm2 and screw holding 41 kg.

Key words : Bagasse, sieving, fraction, glue, particle board.

(Indonesian Sugar Research Journal Vol.XXXIV (2) Juni 1998 :43-50)

Wednesday, October 15, 2008


Hendro Santoso M , Yahya Kurniawan and Edi Purnomo
(Indonesian Sugar Research Institute)


The clarification process of thick juice to reduce non sugar components had been done. Poly aluminium chloride (PAC) and Phosphoric acid were used as clarifying agents in this experiment. Composition of thick juice from a field processor i.e. colour of ICUMSA varied from 11.000 to 18.000 IU, turbidity between 900 to 2000 ppm SiO2 , sludge content between 1,75 to 3,5 g per 100 ml and ash content between 2 to 3 %. The aim of this experiment was to evaluate the potency of PAC (25 – 100 ppm) and Phosphoric acid to clarify of thick juice (50 – 60 brix). The non sugar components such as colour, turbidity, sludge and ash content were reduced by both agents. Sludge content was reduced between 11,56 to 78,9% by PAC and 21,49 to 50,64% by Phosphoric acid treatment. The loss of total sugar was less than 5% for both treatments. PAC could reduced colour of ICUMSA between 0,98 to 17,65%, turbidity 33,03 to 53,54% and ash 1,82 to 12,48%. Furthermore, by using Phosphoric acid treatment it could reduced colour of ICUMSA between 13,07 to 29,73 %, turbidity 36,53 to 47,10 % and ash 11,11 to 12,59 %. Sludge and turbidity removals treated with PAC were higher than that with phosphate. In contrast, PAC treatment in colour reducing of thick juice was lower than phosphate. There was no difference in ash removed for both treatment.

Key words : Polyaluminium chloride, thick cane juice, field processor.

(Indonesian Sugar Research Journal Vol.XXXIV (2) Juni 1998 :32-42)

Wednesday, October 8, 2008

Sucrose-based surfactants (Phase I)


Rosemarie S. Gumera and Blesilda S. Gregorio

The project deals with the production of sucrose-based surfactants from seven (7) types of oil or fatty acids (castor, linseed, soya bean, corn, tallow, oleic acid and hydrogenated palm oil). The process involves saponification for twenty (20) minutes at 105-110 oC and esterfication for two (2) and eight (8) hours at 90-100 oC using two catalyst as potassium and sodium carbonates.

Results revealed that ester level of products obtained from the different oil fatty acids varies depending on the type/amount of oil or fatty acids and catalyst used and esterification time. Only products from castor oil, palm oil and tallow were analyzed for ester content as corn, linseed, soya bean oil yielded a heterogeneous product with excess fatty acid and oleic acid that produced an irritating fume upon sponification thus the decision to discontinue the test run. For palm oil samples treated separately with the catalyst and esterified for two (2) hours, a higher ester value ranging from 161 to 177 mg. KOH per gram sample is attained. Tallow samples esterifies for (2) hours with two catalysts combined, on the other hand, gave a higher ester value (139 and 109 mg KOH per gram sample). Contrary, castor oil samples esterified for eight (8) hours treated with the combined catalyst yielded a higher value.

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