The Use of Na and K-form Strong Acid Cation Resin for Chromatographic Separation of Sucrose from Cane Molasses


Hendro Santoso* and Theresia Hari Sutji W*
*Indonesia Sugar Research Institute
Jl.Pahlawan 25 Pasuruan Indonesia

ABSTRACT

The cane molasses is the residual syrup from sucrose crystallization process. It has sucrose content about 30-40 per cents and reducing sugar about 20-30 percents. Those sugar could be separated from molasses by using Ion Exclusion Chromatographic method. This paper describes the use of Na and K-form strong acid cation resin for separation of sucrose from cane molasses. The experiments was employed on two pilot scale chromatographic column. The A column with diameter of 40 cm and diameter of B column is 25 cm. Both of columns were contained 90 litres of Na-form resin (A column) and 150 litres of K-form resin (B column) with operating condition by flow rate 0,6 and 0,5 bed volum per hour respectively. The feed volume and temperature for both columns were 0,1 bed volume and 70oC. Pol,brix,sucrose, reducing sugar and Total Sugar As Invert (TSAI) were analyzed and evaluated. The results showed that the both of colomns system were improve the quality of sucrose fraction to be separated by increasing the purity of sucrose and decreasing of reducing sugar purity. On the A column system, sucrose purity compare to TSAI could increased up to 65,28% and with the B column system up to 67,77%. Furthere, reducing sugar content decreased up to 30,64% and 48,64% by using A and B column respectively. Sucrose Reducing sugar Ration could achieved 1,72 point by using B column system. It better than A column.

Key words : K and Na-cation resin, chromatography, sucrose fraction, cane molasses.