ETHANOL FERMENTATION OF REDUCING SUGAR FRACTION PRODUCED FROM DESUGARIZATION OF CANE MOLASSES

Aris Toharisman and Hendro Santoso M
(Indonesian Sugar Research Institute)
atoharis@yahoo.com

ABSTRACT

Desugarization of sugar cane molasses using ion exclusion chromatography produces reduction sugar fraction (RSF). The study on the use of RSF as a medium on ethanol production in batch culture using Saccharomyces cerevisiae R5 has been carried out. The overall ethanol yield during 32 hours fermentation with 6,7% initial sugar contents was 3,34% or Yp/s = 0,48 g ethanol/ g sugars. The result was similar to the Yp/s on molasses media with 6,94% initial sugars performed as a control. Other parameter kinetics suh as the specific growth rate of Saccharomyces cerevisiae R5 (mmax), sugar (substrate) up take rate (qs) and ethanol production rate (qp) of both media were also relatively similar. However, the biomass yield (Yx/s) produced on RSF medium was lower than that on molasses medium.

Key words : Desugarization, ion-exclusion chromatography, ethanol fermentation, sugar cane molasses, S.cerevisiae and batch culture.

(Indonesian Sugar Research Journal Vol.XXXIII (4) Desember 1997 :34-39)