USE OF VINASSE, AN ETHANOL DISTILLERY WASTE, AS AN AMENDMENT TO BIOREMEDIATION OF DIESEL OIL CONTAMINATED SOILS


Adriano Pinto Mariano1,3, Sergio Henrique Rezende Crivelaro2,3, Dejanira de Franceschi de Angelis2 and Daniel Marcos Bonotto1
1-Instituto de Geociencias e Ciencias Exatas (IGCE)
2-Departmento de Bioquimica e Microbiologia – Instituto de Biociencias (IB)
3-Programma de Recursos Humanos (PRH – 05) – Agencia Nacional do Petroleo, Gas

Natural e Biocombustiveis (ANP) Universidade Estadual Paulista (Unesp)
Av. 24-A, 1515 – CP 178 – CEP 13506-900 – Rio Claro – SP – Brasil
E-mail: adrianomariano@yahoo.com.br

Abstract.

This work investigated the possibility of using vinasse as an amendment in bioremediation processes by supplying nutrients and as an extra labile carbon source that could increase the microorganisms population. Soil samples were collected at three petrol stations. At two of them, diesel oil spills occurred from underground storage tanks and at the third, samples were collected during the replacement of underground pipes and it was simulated a diesel oil contamination by adding diesel oil purchased from a local petrol station or weathered diesel oil collected from the groundwater of a petrol station. Batch biodegradation experiments were carried out in Bartha biometer flasks that were used to measure microbial CO2 production. Biodegradation efficiency was also measured by quantifying the total petroleum hydrocarbons (TPH) by gas chromatography. Although certain fertilization of the soil and an increase of the microbial population were obtained with the vinasse, in the studied conditions, it demonstrated to not be adequate to enhance the bioremediation efficiency of diesel oil contaminated soils.

Keywords: Bioremediation, Soil, Diesel Oil and Vinasse